Inheritance and Genetic Mapping of Late-Bolting to Early-Bolting Gene, BrEb-1, in Chinese Cabbage (Brassica rapa L.)

Chinese cabbage (Brassica rapa L.) is one of the most important and highly nutritious vegetables in China belonging to Brassicaceae family. Flowering or bolting critical developmental stages flowering plants. For spring-sown cabbage, late-bolting desirable over early-bolting according consumer preferences. We determined inheritance pattern trait using F1 F2 generated from a cross between ‘SY2004’ (late-bolting) ‘CX14-1’ (early-bolting). The genetic analysis revealed that was controlled by an incomplete dominant gene we named BrLb-1. Furthermore, performed bulked segregant (BSA) via whole genome re-sequencing results showed this harbored on chromosome A07 at intersections 20,070,000 25,290,000 bp 20,330,000 25,220,000, interval distance 4.89 Mb. In candidate interval, totals 2321 1526 SNPs with non-synonymous mutations, 229 131 InDels frameshift were found parents pools, respectively. identified three putative genes for trait, including BraA07g029500, BraA07g029530 BraA07g030360, which code AGAMOUS-like MADS-box protein AGL12, pentatricopeptide repeat-containing NAC transcription factor 29, respectively; however, further functional required. These variants could be utilized development molecular markers marker-assisted breeding cabbage.